Probate Fall 08 Omicron Iota step JanuĮditor: The question mark in brackets means that I'm not certain about what the step team chanted. Group does step routines without chanting Guest, Fraternity & Sorority Chants and Songs Īnd say our noses are always in the sky I'LL BE A CONCEITED AKA UNTIL THE DAY THAT I DIE! Okay they say i'm conceited, that is no lie. Looked in the mirror and i SHOOK my pretty head I woke up this morning, jumped out of bed, AKA soror, (Pittsburgh, PA), from private electronic email to Azizi Powell, 8/13/04 posted on 12/2005 (These examples are presented in no particular order.) My sense is that what "is a serious matter" is the sorority itself- its history, its ideals, its present activities, and its members' commitment to that organization. However, a step team may only repeated the words "This is a serious matter this is a serious, serious, serious matter". Each version of this chant includes the refrain "This Is a SERIOUS matter". Most versions of "This Is A Serious Matter" include a reference to the fact that Alpha Kappa Alpha Sorority, Inc was the first Black sorority. This is the date that Alpha Kappa Alpha Sorority, Inc. Many versions of this chant start with the step team saying "1 9 0 8". This chant always includes the line "this is a serious matter". "This is a Serious Matter" is usually composed of two line rhyming verses that state the history of the sorority, praise the sorority and its members, and/or diss (insult) other sororities. Probably that same anonymous commenter also wrote in May 2014 in that discussion thread that "The chant was performed at the Langston University Homecoming Event.". In May 2014 an anonymous commenter who used the screen name "The Author" wrote in the discussion thread of the d iscussion thread for (the.2012 pancocojams post about this AKA chant) that "Serious Matter was authored and first performed at Langston University, Langston Oklahoma in 1986 by the Ivy line lovingly named '15 Girls in Search of 20 Pearls'". Forms of this chant that include the words "This is a serious matter" and its side to side movements with both the palm of both hands stiffly held at the waist and the steppers' head held back stoically paying homage to the sun have become signature parts of the culture of Alpha Kappa Alpha Sorority, Inc. "This Is A Serious Matter" appears to be the one of the most widely known traditional Alpha Kappa Alpha, Inc. Senator Harris may have used this AKA saying before in her campaign and at other times, but I remembered to document her saying it at that event.Ĭlick for a CNN video story entitled "Kamala Harris' secret weapon: Her sorority sisters". and a Democratic candidate for the President of the United States, said "This is a serious matter" in response to a question that she was asked at the 4th Democratic primary debate (October 15, 2019). įor the historical record, Senator Kamala Harris, a member of Alpha Kappa Alpha Sorority, Inc. Much of the content of this post was published on pancocojams in 2012 and also includes references to two comments from that post's discussion thread. *Read the note in the "Information about this chant" section for the identification of the composers of this chant. Thanks also to all those who are quoted in this post and thanks to the publishers of these videos on YouTube. Thanks also to those who shared examples of this chant with me, and all those who are featured in these YouTube videos. Thanks to composers of the Alpha Kappa Alpha Sorority, Inc.* for the chant "This Is A Serious Matter". I also believe that fraternity & sorority songs and chants should only be recited and/or performed by those persons who are affiliated with the specific organization that is associated with that particular chant. I consider fraternity & sorority chants to be cultural artifacts which deserve to be collected, preserved, and studied. The content of this post is presented for cultural, folkloric, and aesthetic purposes. This pancocojams post presents examples of the signature Alpha Kappa Alpha Sorority, Inc.
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Finally, there's Windows corruptions - this is rarer Is hardware troubles - either broken hardware or compatibility #0XED UNMMOUNTABLE BOOT VOLUME DRIVERS#It's been my experience that most STOP errors are caused by 3rd partyĭrivers - either device drivers, or drivers that are a part of a #0XED UNMMOUNTABLE BOOT VOLUME FULL#STOP error numbers can either be the full number (0x0000007B for example), or in "shorthand" (0x7B is shorthand for 0x0000007B). To your system - and Windows is designed to "fail fast" in order to This is done because the potential exists for damage Blue Screen of Death (hence the BSOD acronym)īSOD is an error that causes Windows to STOP (hence the name "STOPĮrror"). Click on the Spoiler (Show/Hide) tag below to show it.Ī BSOD is also known by many other names: The Minidump files (from C:\Windows\Minidump) with your comments.Īdditional content below. If referring to a specific STOP error message, please attach/upload I post as usasma at Bleeping Computer (preferred method of contact) Please notify me if you find anything wrong, missing, new, or just have a comment. Take me to the General STOP message troubleshooting section. Take me to the Specific STOP message troubleshooting section. Take me to the General Information about BSOD's section. Take me to the long Table of Contents listing. Take me straight to the BSOD listing (abbreviated to make looking it up easier). The sheer number of new BSOD KB articles is too daunting to keep up with.Īt last count (23 August 2017) there were more than 554 different STOP error messages in this listing. Some information on Windows NT/2000 links has been left out due to the enormous number of KB articles available.Īs of 02 April 2012, I have discontinued listing Windows 2000 articles.Īs of 21 October 2014, Updates are now limited to adding new BSOD's. Updated for Win 10 (build 1433) on - adding 27 new BSOD's and 3 changed BSOD's Updated for Win10 (build 10586/TH2/1511) on -Īdded 48ish bugchecks (numbers were changed from some of the Win10 Updated for Win10 (build 10240/TH1) on - added 70 bugchecks Updated for Win8.1 on 01Oct2013 - added 23 bugchecks Updated for Win8 on 09May2012 - added 20 bugchecks #0XED UNMMOUNTABLE BOOT VOLUME WINDOWS 7#Initial document from the bugcodes.h file in the Windows 7 SDK v7.0 Debugging Tools For Windows - dated 17 January 2009 Additional information added as it became verifiable on the web. I intend to add these to the table shortly - wich me luck! :0) Last Updated (<- link shows ALL updates):
Not to mention a story so dull and inconsequential that its only purpose is to thread together the game’s twenty-nine levels and explain why the characters transition from snowfields to the insides of an active volcano.īut if you can manage to look past those flaws, you’ll find that Overgrowth is hiding some of the most satisfying combat gameplay I’ve had the pleasure of enjoying this year. #Lugaru in overgrowth fullAnd then there’s the writing chock full of angsty dialogue that gives Shadow the Edgy Hedgy a run for his money. Its platforming controls seem more sadistically interested in watching you faceplant into walls instead of climbing up them. Visually, it suffers from a strange dissonance as its beautifully executed lighting effects clash with textures that belong to a game from Xbox 360 era. For a $30USD game fresh out of early access, a lot of Overgrowth is still seriously unpolished. Of course, it’s far from being any sort of masterpiece. Over and over, I just keep coming back to it so I can indulge in more blood-soaked cotton-tail kicking action! So, you can imagine my excitement when I found out that its sequel, Overgrowth, would finally see an official release after spending nine years percolating in Steam Early Access.Ĭut to a month after its release and, even in the midst of playing Summer’s long-awaited titles and Sonic Forces, Overgrowth the one game I can’t get off my mind. It was brutal, it was fast, it was fun, and only occasionally brought back the traumatic memories of watching Watership Down at an incredibly inappropriate age. Not only was it one of the first indie games of the time to incorporate ragdoll physics, but it also introduced a unique combat system that I’ve still never seen used by another game to this day, revolving entirely around reading context and timing. And it was surprisingly advanced for a 2005 indie game. One demo in particular that has remained lodged firmly in my memory ever since I came across it in my high school years was Lugaru, a kung-fu rabbit fighter developed by one-man team Wolfire Games. Sure, they weren’t the latest gaming hotness of the time, but I still have fond memories of some seriously obscure and underrated gems. As a result, most of my formative gaming years came in the form of magazine-packaged demo discs, which would eventually go on to make up at least eighty-five percent of my game library until 2014. But when I experienced finished playing Therium 2 (The last tale I acquired played) I has been still left in surprise at like a easy game can become so exclusive and impressive.As I’ve mentioned before on this website, the decision between purchasing a new game and affording necessary living expenses like a week’s worth of food and my subscription to Men’s Health magazine (for the articles only, of course) is one that used to plague me up until recent years. The only types of difficulties I have with this game is usually that it'beds a bit leggy adn it has a several pests and the fade to dark when going from objective to objective is certainly a little bit annoying and even can impact a few situations. #Lugaru in overgrowth modI suppose it's price can be a bit very much for such a brief gamé but if you downIoad any various other campaign mods or perform Therium 2 I feel like the purchase was acceptable that and aIl of the móds are usually free.Mainly because very long as there are mod creators for this gamé I will continually be arriving back again. I'have always been heading to give my Total opinion on this gamé: When it arrives to tale and recording the evil of battle and dislike this game displays it in a neat three to four hr game (Like Lugaru tale), It is definitely a little bit laggy and can disturb from time to period but despite thát this game is definitely one of the greatest little indie games I possess played. #Lugaru in overgrowth PcThis could be a game with a third person, likeFree pc games Assassins Creed. #Lugaru in overgrowth for free
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If you have any question, send it to us and we’ll attempt to answer it here. This page attempts to answer all your frequently asked questions about bed bath and beyond coupons and shopping at BB&B. Liu C, Neale ZG, Cao G (2016) Understanding electrochemical potentials of cathode materials in rechargeable batteries. Ionics 23(3):497–540įeng K, Li M, Liu W, Kashkooli AG, Xiao X, Cai M, Chen Z (2018) Silicon-based anodes for lithium-ion batteries: From fundamentals to practical applications. Nano Energy 31:113–143Īrya A, Sharma A (2017) Polymer electrolytes for lithium ion batteries: a critical study. Zuo X, Zhu J, Müller-Buschbaum P, Cheng Y-J (2017) Silicon based lithium-ion battery anodes: a chronicle perspective review. Su X, Wu Q, Li J, Xiao X, Lott A, Lu W, Sheldon BW, Wu J (2014) Silicon-based nanomaterials for lithium-ion batteries: a review. The capacity retention of the silicon component in the powder mixture dropped below 60% after only one cycle, whereas silicon in the composite still has about 70% remaining capacity after 100 cycles. Owing to the presence of the robust carbon shell and internal void space, the yolk-shell composites exhibited significantly better cycling stability than the powder mixture of silicon and graphite. The synthetic phenol-formaldehyde resole resin was used to bind the graphite to the surfaces of the aluminum-silicon alloy particles, and then was thermal cross-linked and carbonized aluminum was gently dissolved into ferric chloride etchant, leaving void space between the carbon shell and silicon core. A low-cost method is developed to prepare yolk-shell composite particles in this study. However, its commercial application is largely limited by the poor cycling stability due to its huge volume change during lithiation and delithiation. Silicon is an attractive anode material for lithium-ion batteries due to its ultrahigh theoretical specific capacity. After an argument in which Ivan’s parents threatened physical punishment, Salalisk lashed out to protect the boy - and soon after, the odd pair ran from home, never looking back. "Mistreating boy and beast alike, believed the monster would grow up bloodthirsty and that their child was a fool for trusting a wild creature. You'll encounter these pairings roaming the roads, mountains and forest of Crown Island, and each Tamer has a special backstory and relationship with their monster. Ivan has grown alongside the monster for as long as he can remember, and often Salalisk has been his only comfort in a home with his strict and often neglectful parents." Having found an egg when he was a small child, his parents threw the egg into the fireplace to dispose of it - only for it to hatch into a strange monster. "Ivan and his Salalisk are an odd-looking pair, but the teenage boy and his monster are inseparable. A Pivotal Choice that Drastically Changes the Ending and Post-Gameīackers will receive a link to our Private Backer Demo/Beta.This brand new species is totally yours - even to name.Īs you play online, trading and battling, your creations could reach across the globe! In Monster Crown, the offspring are true mixes of both parents, in every way. Unfortunately, we never felt like the offspring was a true crossbreed of both parents - It wasn't a brand new species. The idea of combining the Monsters we bonded with during our adventures to create amazing new monsters got us so excited. Something that always drew us in was Monster Breeding. Exploring these amazing worlds, packed to the brim with awesome monsters lit our imaginations on fire. As kids, we loved playing 90s Monster Catching/Taming games. More recently, studies have shown that MALS can also be used for quantification of VLPs in solution 19, 20. MALS has been widely used in tandem with SEC or field flow fractionation to determine the absolute molecular weight, size, conformation, and distribution of polymers and protein biotherapeutics 16, 17, 18. Further, the utility of SEC can be enhanced by combining it with multiangle light scattering (MALS) and refractive index (RI) detectors. SEC is widely used as a polishing purification step of viral vectors and has been developed as a characterization method for virus-like particles (VLPs) and influenza particles 11, 12, 13, 14, 15. However, SEC has the advantage of separating monomeric AAV capsids from higher-order aggregates and other extrinsic impurities. Size exclusion chromatography (SEC), which separates species in solution by size, similarly uses a UV-based detection method. Protein and nucleic acid impurities were found to significantly impact titer results, limiting the use of this method for only highly purified samples. Although OD titers were less variable, the bulk-nature of OD measurements did not allow correction for the impact of extrinsic capsid impurities or aggregates on UV absorbance. In 2003, a bulk OD method to determine AAV capsid (Cp) and vector genome (Vg) titer was proposed as an alternative to qPCR and ELISA. Despite their historic and continued use, each method has its own limitations, such as low throughput or high variability 7, 8.īulk optical density (OD) measurements have been used to quantify the vector genome and capsid protein content of adenovirus preparations 9, 10. Various methods, including electron microscopy, dynamic light scattering, analytical ultracentrifugation, ELISA, and PCR are used to independently monitor purified AAV size, aggregation propensity, stability, empty-to-full capsid ratio, capsid (Cp) and vector genome (Vg) titer, respectively. Therefore, a successful gene therapy program depends on accurate vector characterization and titration, which dictates the safety and efficacy in humans. Previous gene therapy programs have shown positive correlation in general between the presence of gene copy numbers (vg/kg patient weight) and protein expression 5, 6. While several successful methods exist for large-scale, clinical AAV production 2, 3, 4, the development of robust analytics to assess variables in the overall process development and final AAV drug products is an iterative process. As of April 2020, 244 gene therapy trials using AAVs are ongoing worldwide, 24 of which are in Phase III clinical trials, ( ). Due to their relative safety and long-term gene expression, multiple serotypes of recombinant AAV vectors are in use across gene therapy programs at the clinical and research stage 1. AAV particles consist of a small ~ 25 nm icosahedral capsid composed of three types of structural proteins (VP1, VP2, and VP3) coating a single-stranded ~ 4.7 kb genome. These novel applications designate this well-established method as a powerful tool for product development and process analytics in future gene therapy programs.Īdeno-associated virus (AAV) belongs to the genus Dependoparvovirus in the family Parvoviridae. #DYNAMIC LIGHT SCATTERING PROTOCOL SERIES#In the current study, a new series of equations for SEC-MALS are used in tandem with intrinsic properties of the capsids and encapsidated DNA to quantify multiple physical AAV attributes in one 20-min run with minimal sample manipulation, high accuracy, and high precision. Currently, multiple techniques are required to generate this information, with varying accuracy and precision. The current study demonstrates that this method provides detailed AAV characterization information, including but not limited to aggregation profile, size-distribution, capsid content, capsid molar mass, encapsidated DNA molar mass, and total capsid and vector genome titer. Size exclusion chromatography (SEC) coupled to multiangle light scattering (MALS) offers a straightforward approach to comprehensively characterize AAV capsids. Complete and precise characterization of capsid particles, including capsid and vector genome concentration, is necessary to safely and efficaciously dose patients. Adeno associated virus (AAV) capsids are a leading modality for in vivo gene delivery.
It’s somewhat buggy, as one might expect from a smaller project, but it’s an intriguing game with a really interesting gameplay loop. It’s a fine experience an one that just about any fan of these kinds of games should enjoy trying out. It’s quite dated looking, but its atmosphere, pacing, and general gameplay mood allow the game to thrive for all of the right reasons. It also comes with some serious graphical limits, which one might expect from the small development team that worked on it. It was also praised for having a more panicked environment than other horror games such as Warehouse 13. SCP CONTAINMENT BREACH DOWNLOAD SITE DOWNLOADSCP - Containment Breach Trailer 2 Watch on DOWNLOAD SCP - Containment Breach v1.3.11 (235 MB) MIRROR 1 () MIRROR 2 (MEGA) Changelog. SCP CONTAINMENT BREACH DOWNLOAD SITE FREEUpon release, the general mannerism around the game was one of positivity – many appreciated the angles it tried to take. SCP - Containment Breach SCP - Containment Breach is a free survival horror game based on the works of the SCP Foundation community. A solid horror offering with some obvious limits The whole pace of the game gradually changes as you move through as you reach the end of the procedurally generated facility, you will see things get more frantic and fearsome. You can also find other SCPs who might be more willing to help you escape the place. This includes tools such as gas masks, batteries, and various keycards. This creates a terrifying environment that involved other creatures trying to escape whilst the officers try their best to get everyone back in their cages.Īs the game opens up, you find numerous items to help you find it easier to survive. When within range of the composition, forcibly draws the victim towards itself, making them cut their wrist in an attempt to finish it using their own blood. As you try to escape, though, you’ll see the various other “SCPs” trying to escape, too. A prion which causes its victims to turn into zombies. The facility you are in is in a state of total disarray, and your goal is simple – get to the exit and make away from the facility. begins to malfunction, causing a site-wide containment breach to occur. SCP CONTAINMENT BREACH DOWNLOAD SITE APK MODThe game itself has a pretty simple process – you play as ‘Class-D’, a form of test subject. Download SCP - Containment Breach Mobile(No Ads) 1.2.0 APK mod for Android, com. SCP - Containment Breach is a game that I had so much fun with I really cannot recommend this game highly enough and it is free While the start and the. How good, then, can SCP be? Supernatural horror on a budget It’s terrifying, engaging, and highly exciting – but it’s also got some limits. Taking part in the body of a human test subject who has been stored away, you need to try and break out from the SCP facility and escape. 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The teen hasn’t had an easy life: in addition to being born without hands, he has only one leg and spent much of his young life in an orphanage for boys. #RUSSIAN PIANO PRODIGY MOVIE#He learned basic songs almost immediately, and was soon playing everyday, teaching himself and playing along to movie soundtracks.Īlexey does have prosthetic hands but never uses them when he plays the piano, because he says they’re uncomfortable for moving around the keys. Alexey has always enjoyed the piano music of Mozart and Vivaldi, but didn’t take up the instrument until a friend helped him find different notes on the keys. The teenager has been playing the instrument for less than three years and already performed in concert halls and on TV, proving that natural talent can’t be held back by a handicap. Then, you realize that the Russian teen is playing without any fingers, and your jaw pretty much drops to the floor. 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